منابع مشابه
Permanganate Fixation of Plant Cells
In an evaluation of procedures explored to circumvent some of the problems of osmium tetroxide-fixation and methacrylate embedding of plant materials, excised segments of root tips of Zea mays were fixed for electron microscopy in potassium permanganate in the following treatment variations: unbuffered and veronal-acetate buffered solutions of 0.6, 2.0, and 5.0 per cent KMnO(4) at pH 5.0, 6.0, ...
متن کاملSodium Permanganate Fixation for Electron Microscopy
Heightened interest in the cytology of certain free-living flatworms has been evident in the literature throughout the past year (5, 6, 10-12). The variety of problems dealt with by various investigators reflects the abundance of curious cellular specialization found in this group of animals. Moreover, the suitability of the Turbellarians for the study of regenerative processes is well recogniz...
متن کاملA Study of Potassium Permanganate ' Fixation ' for Electron Microscopy
The action of buffered potassium permanganate as a fixative for electron microscopy has been investigated. Electron contrast has been shown to be produced by the deposition of granular material in the tissue. The particles are about 50 A in diameter. Actual fixation of the tissue is performed by the dehydrating alcohol. Histochemical studies have shown that RNA and histones are removed, whereas...
متن کاملDifferentiation of Plant Cells During Symbiotic Nitrogen Fixation
Nitrogen-fixing symbioses between legumes and bacteria of the family Rhizobiaceae involve differentiation of both plant and bacterial cells. Differentiation of plant root cells is required to build an organ, the nodule, which can feed and accommodate a large population of bacteria under conditions conducive to nitrogen fixation. An efficient vascular system is built to connect the nodule to the...
متن کاملPermanganate Fixation of the Golgi Complex and Other Cytoplasmic Structures of Mammalian Testes
Observations on the fine structure of KMnO(4)-fixed testes of small mammals (guinea pig, rat, and mouse) reveal certain morphological differences between the spermatogenic and Sertoli cells which have not been demonstrated in the same tissue fixed with OsO(4). Aggregates of minute circular profiles, much smaller than the spherical Golgi vesicles, are described in close association with the Golg...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1959
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.6.3.431